Job ID 12456 Location Case Main Campus Full/Part Time Full-Time Regular/Temporary Regular Job Description POSITION OBJECTIVE Working under general supervision, the Research Assistant 2 will plan and carry out projects in accordance with general plans. The lab performs advanced research at the intersection of cancer biology, intracellular signal transduction, and membrane trafficking. The research assistant will join a friendly, pleasant lab group that pursues cutting-edge biomedical research to understand the mechanism of Golgi trafficking, its regulation, and contribution to human disease, especially cancer. The lab is using discoveries to develop new approaches to cancer treatment. The position will include working with mice. ESSENTIAL FUNCTIONS Plan and carry out project in accordance with general plans. Conduct a series of experiments to supply scientific information as part of a larger project. Perform experiments using PCR, gel electrophoresis, Western blotting, confocal microscopy, and basic molecular biology and protein biochemistry. May assist in developing improved techniques, projection methods or procedures. (20%) Collect and analyze data using microscopy, chemiluminescence, and related methods. Analyze data using spreadsheet software, image analysis software, and similar computational methods. Prepare graphs, charts, and slides to report experimental data. (10%) Organize and maintain mouse colony, keep records of mouse lines, genotype and wean pups. (20%) Perform laboratory maintenance and organization under general guidance. Track and maintain supply inventory, work with fund manager, laboratory personnel, and supply company representatives to ensure supplies are ordered cost effectively and in a timely manner and on correct funding source. Help maintain laboratory equipment in good working order, appropriately calibrated and maintained. Ensure a clean, orderly, safe working environment. Maintain records of laboratory stocks and reagents, equipment manuals and procedures, purchases received and outstanding, packing slips, and storage destination, and laboratory experimental procedures and data. (33%) Train new staff; direct the work of students. Coordinate laboratory safety with the department of occupational and environmental safety. Ensure compliance with safety protocols, orient new personnel and direct them to obtain appropriate training through the department of occupational and environmental safety, maintain records of personnel training, work with the department of occupational and environmental safety to ensure compliance with rules and regulations regarding chemical, biohazard, radiochemical, and general laboratory hazards. (10%) NONESSENTIAL FUNCTIONS 1. Attend and participate in laboratory meetings. (2%) 2. Perform other duties as assigned. (5%) CONTACTS Department: Daily contact with supervisor and lab members to discuss research and maintain workflow. University: Occasional contact with Environmental Health and Safety to ensure safety compliance. External: Regular contact with supply and equipment vendors to obtain quotes and to ensure cost effective purchasing and maintenance of laboratory equipment. Students: Daily contact with postdoctoral and student employees to provide orientation to the lab, explain policies and procedures, coordinate purchasing, and collaboration on experiments. SUPERVISORY RESPONSIBILITY This position has no direct supervision of staff employees. Train new staff; direct the work of students. QUALIFICATIONS Education/Experience: Bachelor of Science degree and 1 to 3 years of related experience or Associate's degree in an approved biotechnology program and 2 to 4 years of experience required. REQUIRED SKILLS Has knowledge of commonly used concepts, practices, and procedures within a biomedical