Job ID 12484 Location Case Main Campus Full/Part Time Full-Time Regular/Temporary Regular Job Description POSITION OBJECTIVE Working under limited supervision, perform development work on a federally funded project to develop engineered cartilage sheets and biological bone material for implantation in animals and humans. The Research Assistant 3 will coordinate research activities to design, implement, test, and validate data collection and analysis methods in cell biology. This position will workwith animal and human tissue. ESSENTIAL FUNCTIONS 1. Coordinate laboratory and research activities. Perform development work, including isolation of cells from human/animal tissues, cell expansion and biomaterial seeding, tissue culture,biochemical assays, immunofluorescence performance and analysis, and flow cytometry performance and analysis. (25%) 2. Work closely with the principal investigators in managing tissue engineering research, resolving questions, and correcting scientific errors. Design and manage individual experiments, reporting these in a consistent fashion and in a timely manner, managing issues of the scientific method including positive and negative controls, reproducibility, sample size estimate valuation, trends and troubleshooting, and refining techniques into standard operating procedures in the laboratory. (20%) 3. Carry out complex research assignments of a non-routine nature. Specific experimental activities will require the ability to perform long term bioreactor expansion of human and animal cells and maintain bioreactor systems. Maintain accurate records of the data obtained. Compile and analyze the data obtained. (10%) 4. Devise new protocols and techniques for research projects involving a high degree of skill and training. These include characterizing the methods for isolation of cells from human /animal tissues, cell expansion and biomaterial seeding. (9%) 5. Evaluate adequacy of techniques and provide feedback on experimental design. Recordresults using appropriate software, such as Excel. Prepare and present results to supervisors. Optimize and troubleshoot procedures to comply with expected outcomes. (8%) 6. May supervise students and technicians in the completion of experiments and compilation and reporting of results. (6%) 7. May co-author research projects in support of the principal investigators as the outcomes and circumstances of the program permit. (6%) 8. May monitor the laboratory budget. Monitor supply needs and consumption and communicate re-order requirements to project administrators. (6%) OTHER FUNCTIONS 1. Maintain lab equipment and proper lab operation. (4%) 2. Prepare solutions, buffers, culture media, and maintain liquid nitrogen tanks, CO2 tanks, and incubators etc. (3%) 3. Perform other duties as assigned. (3%) CONTACTS Department: Daily contact with faculty and staff in the departments of Biology and Chemical and Biomolecular Engineering who are working in the group to maintain workflow. University: Regular contact with faculty and staff in the departments of Biology, Mechanical and Aerospace, and Civil Engineering to maintain workflow. External: Frequent contact with external collaborators (industrial and academic) for experimental planning and collaborative efforts. Students: This position entails infrequent contact with student employees. SUPERVISORY RESPONSIBILITIES This position has no direct supervision of staff employees. The employee may be asked to train new hires. QUALIFICAITONS Experience: 3 to 5 years of related experience required. Education: Bachelor of Science degree required. REQUIRED SKILLS 1. Experience with aseptic protocols and procedures involving human and/or animal cells. 2. Ability to keep accurate and up to date information. 3. Ability to operate common laboratory equipment including